De acordo com o fabricante a Protei

De acordo com o fabricante a Proteinase K deve ser diluída em 50 nM de Tris-HCL (ph 7,5), contendo 5 nM de de cloreto de cálcio e 50 % (v/v) glicerol. A solução de Tris-HCL (ph 7,5) é uma solução caseira que pode ser feita no laboratório, preciso saber se a molaridade dele pode ser 1 M ( Tris-HCl 1 M ph 7,5). Outra dúvida é quanto as medidas de cada solução que está em nM e diz que a solução deve conter 50% de Glicerol. Preciso saber a medida em ml/gr de cada solução e como é feita essa mistura das soluções.

Resultados (Inglês) 1:

According to the manufacturer the Proteinase K must be diluted in 50 nM Tris-HCL (ph 7.5) containing 5 nM for calcium chloride and 50% (v/v) glycerol. The solution of Tris-HCL (ph 7.5) is a homemade solution that may be made in the laboratory, I need to know if the Molarity of it can be 1 M (1 M Tris-HCl ph 7.5). Another question is how each solution measures are in nM and says that the solution must contain 50% glycerol. I need to know the measure in ml/g of each solution and how is this mix of solutions.

Resultados (Inglês) 2:

According to the manufacturer Proteinase K should be diluted in 50 mM Tris-HCl (pH 7.5) containing 5 nM calcium chloride and 50% (v / v) glycerol. The Tris-HCl solution (pH 7.5) solution which is a home can be made in the laboratory must know whether it can be molarity 1 M (1 M Tris-HCl pH 7.5). Another question is how the measurements of each solution that is in nM and says that the solution must contain 50% glycerol. I need to know the measure in ml / g of each solution and how this mixture of solutions made.

Resultados (Inglês) 3:

In accordance with the manufacturer to proteinase K must be diluted with 50 nm of Tris HCl (pH 7.5), with 5 nm of calcium chloride and 50% (V / V) glycerol. The solution of Tris HCl (pH 7.5) is a national solution that can be made in the laboratory, I need to know if the molarity of him may be 1 m (1 m Tris HCl, pH 7.5).Another question is how the measures of each solution is in nm and says that the solution should contain 50% of glycerol. I need to know the extent ml / g of each solution and how it is done that mixing of the solutions.